The blood does not come into contact with the environment or with the operator, let alone with the instrument. At the end of the centrifugation, the samples are analysed in two phases: the lower sediment part features red and white blood cells. The upper part features plasma and platelets with growth factors. By using a special pipette, the PRP is suctioned and a chemical activator is added to degranulate the platelets, facilitating the outflow of growth factors. The prepared PRP may then be injected into the scalp by using a sterile syringe.
The operations must be consecutive, given that the platelets have a short lifespan. The blood must be drawn from the individual who is to be treated. The preparation, which lasts around 45 minutes, is carried out by specialized clinical personnel or hematologists. Thus, no risks are present in this treatment. The centrifugation does not alter the blood cells and is used solely to separate them. If the entire procedure carried out by qualified personnel is conducted in a designated environment, there is no risk of contamination.

As far as results in hair loss treatments are concerned, the growth factors stimulate the bulb cells (of the dermal papilla, matrix and melanocytes) to keep the anagen phase active, produce the hair shaft, reduce inflammatory processes in the skin and reduce cellular death. Moreover, the extremely high concentration of natural growth factors is able to stimulate stem cells in the remaining bulbs to once again generate a hair bulb. Thus, in the case of pattern hair loss, this procedure can lead to the regrowth of a number of hairs (obviously not quantifiable beforehand), especially in the early stages of the illness (in particular from stages I to IV of the Hamilton scale and in stages I to II of the Ludwig scale).
According to scientific literature, significant growth is seen in 60-70% of cases. On average, 2-4 sessions are performed over the course of 3 months; in alopecia areata (spot baldness), this may well be the most important treatment technique in terms of hair regrowth percentages in active patches. In scarring hair loss, it may have a significant effect on inflammation control and for decreasing the progression of active hair bulb death, especially in early forms. If residual hair follicle stem cells exist in the dermis, they may also be stimulated.

PRP therapy can be considered one of the most advanced and effective therapy techniques in the trichological field. Success depends, however, on the type of illness, on various concurrent situations and on the presence or lack of stem cells that may be activated. The most advanced theories now tend to use a treatment with PRP before, during or after the micro-autotransplant operation, in order to obtain:
– faster implantation of transplanted bulbs
– an increase in the bulb implantation percentage
– growth stimulation of silent bulbs
– faster healing of the scalp treated with the hair transplant.